Transfection Reagents
Andwin Scientific INTERFERIN SIRNA TRANSFECT 1ML
The INTERFERin siRNA Transfection Reagent guarantee reproducible transfection, and attain over 90% silencing in a broad variety of cells. A 1 milliliter volume of this reagent is enough to perform 500 to 1000 transfections in 24-well plates. This product can be used with serum and antibiotics. It must be stored at 4C (39F). This product is sold individually in a quantity of 1 mL.
Kyfora Bio PEI 25K™- Transfection Grade (Polyethylenimine, Linear, MW 25000) 1GR
502559826 POLYETHYLENIMINE LINEAR
Kyfora Bio PEI MAX - Transfection Grade (Linear Polyethylenimine Hydrochloride MW 40,000) 100MG
Kyfora Bio’s Polyethyleneimine MAX (PEI MAX) is a powerful, reliable, and cost-effective reagent, widely regarded as the gold standard for both in vitro and in vivo applications. PEI MAX can be used to produce high viral, recombinant protein, and monoclonal antibody titers across a diverse array of cell lines, including HEK293 and CHO cells. It offers exceptional transfection efficiency for both stable and transient transfections in adherent and suspension cultures, all while preserving cell viability, making it an ideal transfection reagent compared to other products on the market.
Kyfora Bio DMG-PEG2000 500 MG
DMG-PEG 2000 (1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000) is a PEGylated lipid used in the formation of lipid nanoparticles (LNP) for the development of mRNA vaccines such as COVID-19 vaccine and for the delivery of nucleic acids such as RNAis. It is derived from dimyristoyl glycerol (DMG) which behaves similar to phospholipids, namely in the way they associate with membranes. PEGylation of the lipid enhances bioavailability and prevents opsonization of the LNP. This product is for research use only. Key application(s): mRNA vaccine, gene delivery
New England Biolabs, Inc. Universal miRNA Cloning Linker – 5 µg
Linker/RNA Ligation Protocol: The Universal miRNA cloning linker has a 3' blocking group (amine) to prevent self ligation, circularization and ligation to RNA at the 5' end. It also contains the BanI restriction site compatible with the miRNA cloning protocol used by Bartel. For ligation, resuspend the lyophilized oligonucleotide in RNAse-free water to 100 M stock concentration. (A resuspension volume of 8.6 µl will give a 0.58ug/l or 100 µM stock solution). Ligations are carried out with T4 RNA Ligase I in the absence of ATP. Approximately 1 ug is typically used per reaction.
New England Biolabs, Inc. NEB Turbo Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
Chemically competent E. coli competent cells suitable for high efficiency transformation and rapid colony growth.
Agilent Technologies BL21(DE3) COMPETENT CELLS
BL21(DE3) Competent Cells shows high-level protein expression, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Used for non-toxic proteins.